A Secret Weapon For thr77
A Secret Weapon For thr77
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which leads to chromosome instability, and therefore may well add to tumorigenesis. This protein was found to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial function in advertising mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be explained. Two more splice variants have been described but comprehensive nucleotide sequence information and facts isn't nevertheless accessible.
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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide ordinarily contains the epitope recognized by the antibody.
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This information outlines more achievable optimization of cellular and immunoassay parameters to be sure the very best results are...
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This guideline outlines further achievable optimization of cellular and immunoassay parameters to be sure the absolute best effects are...
Antibodies sure to the blocking peptide no longer bind into the epitope about the target protein. By comparing the staining in the blocked antibody compared to the antibody on your own, you can see which staining is particular.
Focus on Information and facts Cyclin E belongs towards the extremely conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show unique expression and degradation styles which lead to your temporal coordination of each and every mitotic celebration. Cyclin E varieties a fancy with and features as being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates at the G1-S phase boundary and it is degraded as cells development via S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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